Study of Genetic Diversity in Wheat (triticum Aestivum L.) Using Random Amplified Polymorphic Dna (rapd) Markers

Twelve wheat genotypes developed through hybridization programme were screened for genetic diversity through RAPD marker. A total of 102 loci were amplified with 14 primers out of which 91 (89.2%) were polymorphic and only 11(10.8%) were monomorphic. Fragments size ranged from 142bp-5.3kb and fragments produced by various primers ranged from 1-11 with an average of 7.1 fragments per primer. The highest number of loci (13) was amplified with primer A10, while the lowest number (1) with primer B-10. Results revealed that variety SARC-1, PKV1600 and Chakwal-86 contain a specific segment of 478 bp while SARC-1 contains another specific segment of 957 bp amplified with primer A-07. Genetically most similar genotypes were SARC-1 and Chakwal-86 (70%) while most dissimilar genotypes were Sarsabz and PKV-1600 (33%). On the basis of results achieved, the varieties could be divided into 3 groups, Kiran-95, Marvi-2000 and Sarsabz in one group, Bhitai, ESW-9525, Inqilab-91, Khirman and Abadgar-93 clustered in second group and SARC-1, Chakwal-86, PKV-1600 and CM 24/87 in the third group.